Selective Incorporation of ~(19)F-Labeled Trp Side Chains for NMR-Spectroscopy-Based Ligand-Protein Interaction Studies

摘要

In the last few years NMR spectroscopy has increasingly been used in pharmaceutical drug discovery and development as a result of its ability to provide structural and dynamic information at atomic resolution. However, the attainment of structural information on protein-ligand complexes by NMR spectroscopy is limited to small to medium size proteins (MW < 30 kDa) and by the lengthy nature of the protein resonance assignment process.