Qualitative and quantitative analysis of gallic acid in Alchemilla vulgaris, Allium ursinum, Acorus calamus and Solidago virga-aurea by chip-electrospray ionization mass spectrometry and high performance liquid chromatography

摘要

This study presents the results obtained from qualitative and quantitative analysis of gallic acid from hydro-alcoholic extracts (methanol, ethanol) of plants from Plantae regnum. Plant qualitative analysis was performed using a novel mass spectrometric (MS) method based on fully automated chip-nanoelectrospray ionization (nanoESI) high capacity ion trap (HCT) while quantitative analysis was carried out by high performance liquid chromatography (HPLC). These methods were applied to Alchemilla vulgaris - common lady's-mantle (aerial part), Allium ursinum - bear's garlic (leaves), Acorus calamus - common sweet flag (roots), Solidago virga-aurea - goldenrod (aerial part). Obtained results indicated that methanol extracts (96%, 80%) have a gallic acid content ranging between 0.0011 - 0.0576 mg mL~(-1) extract while the ethanol extracts (96%, 60%) exhibit a gallic acid concentration that varies between 0.0010 -0.0182 mg mL~(-1) extract.