Genetic purity testing of loose-curd cauliflower hybrids using SSR markers and grow out test.

摘要

Loose-curd cauliflower is a special style of cauliflower and widely cultivated in southeast China. To ensure the supply of good quality hybrid seeds to the market, a commercially important loose-curd cauliflower cultivar derived from two double haploid (DH) lines was subjected to genetic purity assessment using a grow out test (GOT) and Simple Sequence Repeat (SSR markers). The DNA bulks of female and male parents were detected with 96 pairs of SSR primers and seven pairs exhibited polymorphism. Two primers producing suitable male and female specific markers were chosen for purity testing of the F₁-hybrid seeds. The purity level determined by SSR markers was then compared with the GOT performed on the same seed lot. Our study demonstrated that SSR markers were more reliable, accurate and efficient than the commonly used GOT. This is the first report demonstrating the genetic purity testing on hybrids derived from DH lines using molecular markers.