The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars

摘要

Application of in vitro plant biotechnology in berry crops depends on the availability of efficient regeneration protocols that are specific to the genotype background and to the correct combination of exogenous hormones (auxin and cytokinin) added to the medium. Strawberry regeneration protocols are available for different cultivars, while more limited information are available for blueberry cultivars. In this study, we show that the best regeneration efficiency for the leaves of strawberry cultivar Calypso were obtained culturing in a medium supplemented with thidiazuron (TDZ) 0.5 mg L-1 and 2,4-dichlorophenoxyacetic acid (2,4-D) 0.02 mg L-1. The best regeneration efficiency for cultivar Sveva leaves was obtained culturing in a medium supplemented with N6-benzyladenine (BA) 3 mg L-1 and indole-3-butyric acid (IBA) 0.2 mg L-1.