Pathomorphology and PCR diagnosis of ovine pulmonary adenomatosis

摘要

Ovine pulmonary adenomatosis (OPA) is a transmissible lung neoplasm of sheep induced byJaagsiekte sheep retrovirus (JSRV). One hundred ten lung samples from slaughtered sheeppresented focal nodular or diffused consolidated OPA in single or multiple lobes. Both formshad acinar and papillary patterns of alveolar & bronchiolar epithelial proliferation lined bycuboidal to columnar epithelial cells, myxoid nodules, extensive caseation necrosis,peribronchiolar and perivascular lymphoid hyperplasia with interlobular septal thickening.Numerous alveolar macrophages but few neutrophils and mononuclear cells were present. AJSRV specific hemi-nested PCR (hn PCR) from DNA samples produced amplified product (176bp) in OPA samples confirmed in primary and secondary PCR’s (133 bp), and samples kept in10% formalin after 45 days. Sensitivity of U3 hn PCR in DNA samples was detected up to 10 7fold dilutions in both rounds.