Preparation of Modified Magnetic Nano-Fe3O4 Chitosan/ Graphene Oxide for the Preconcentration and Determination of Copper (II) ions in Biological and Environmental Water Samples Prior to Flame Atomic Absorption Spectrometry

摘要

A simple, highly sensitive, accurate and selective method for determination of trace amounts of Cu~(2+) in water samples .In this paper, chitosan grafted with graphene oxide sheets showed an increased surface area was used to encapsulate nano-Fe3O4 and produce a nano-Fe3O4-encapsulated-chitosan/ graphene oxide sorbent based new sorbent was prepared. Flame atomic absorption spectrometer was utilized for determination of Cu~(2+).Some of the important parameters on the preconcentration and complex formation were selected and optimized. Under the optimized conditionsthe limit of detection (LOD) and limit of quantification (LOQ)were 0.30,0.750 and theproposed methodhas a good reproducibility 0.90% (RSD %).The enrichment factor was 200 and the percentage of recovery was in the range of 95-100% The method was successfully applied to the recovery of Cu~(2+) in different type of water samples. Graphene oxide and its derivatessuch as magnetic nano-Fe3O4-encapsulated-chitosan/graphene oxide in this study is full of potential to use as an excellent adsorbent in the extraction method like solid phase extraction(SPE) and solid phase micro extraction(SPME). In the present study, we report the application of preconcentration techniques still continues increasingly for trace metal determinations by flame atomic absorption spectrometry (FAAS) for quantification of Cu~(2+) in Formalin-fixed paraffin-embedded (FFPE) tissues from Liver loggerhead turtles.This method exhibits the superiority in compared to the other adsorption reagents because of the fact that there is no necessity of any complexing reagent and optimum pH of solution presents in acidic media.