COMPARISON OF BIOETHANOL PRODUCTION FROM ACID HYDROLYZATES OF WASTE OFFICE PAPER USING SACCHAROMYCES CEREVISIAE AND SPATHASPORA PASSALIDARUM

摘要

The aim of this work was to evaluate waste office paper as raw material for bioethanol productionusing four strains of Saccharomyces cerevisiae and Spathasporapassalidarum HMD 14.2.Waste paper was hydrolyzed with 1-5% V/V sulfuric acid to 2-10% w/Vbiomass load for 60-120 min. The most significant variable for the total reduced sugar (TRS) was the biomass load, followed by the acid concentration. The pretreatment time did not exert any significant effect on TRS. The hydrolysate obtained with 5% V/V sulfuric acid, 10% w/V biomass load and 1 hour, containing8.45 g/L glucose and 9.27 g/L xylose, was chosen for the fermentations. The fermentation with S. passalidarum resulted in higher ethanol formation (3.54 g/L) than the fermentation with S. cerevisiae, which corresponds to a hypothetical yield of 0.708 g/g glucose. This indicates that S. passalidarum produces ethanol not only from glucose, but also from xylose.