首页> 外文期刊>Research in Microbiology >Secretory aspartyl peptidase activity from mycelia of the human fungal pathogen Fonsecaea pedrosoi: effect of HIV aspartyl proteolytic inhibitors.
【24h】

Secretory aspartyl peptidase activity from mycelia of the human fungal pathogen Fonsecaea pedrosoi: effect of HIV aspartyl proteolytic inhibitors.

机译:人类真菌病原体Fonsecaea pedrosoi菌丝体分泌的天冬氨酰肽酶活性:HIV天冬氨酰蛋白水解抑制剂的作用。

获取原文
获取原文并翻译 | 示例
       

摘要

Fonsecaea pedrosoi is the principal causative agent of chromoblastomycosis, which is a chronic, often debilitating, suppurative and granulomatous mycosis. Very little is known about the hydrolytic enzymes produced by this human fungal pathogen. In the present study, we have identified extracellular proteolytic activity from F. pedrosoi mycelial forms when grown in chemically defined conditions. Secretory aspartyl peptidase activity was measured during 15 days of fungal growth in vitro using bovine serum albumin (BSA) as the soluble substrate and extreme acidic pH (2.0). This activity was totally inhibited by pepstatin A, a classic aspartyl peptidase inhibitor. Conversely, metallo (o-phenanthroline), cysteine (E-64) and serine (PMSF) proteolytic inhibitors failed to restrain proteolytic activity. We also evaluated the effect of four distinct HIV aspartyl peptidase inhibitors on the secretory proteolytic activity of F. pedrosoi mycelia. Indinavir, ritonavir and nelfinavir powerfully inhibited extracellular aspartyl proteolytic activity by approximately 97, 96 and 87%, respectively, whereas saquinavir did not significantly interfere with BSA hydrolysis. Mycelial-derived secretory aspartyl peptidase activity cleaved other proteinaceous substrates, including human albumin, fibrinogen, fibronectin, laminin and type I collagen. As previously reported by our group, conidia also produce secretory aspartyl peptidase. In this sense, we investigated the effect of pepstatin A on F. pedrosoi development. Pepstatin A was able to inhibit the growth of conidium and its transformation into mycelium. Taken together, our results suggest a possible participation of aspartyl peptidases in the essential fungal processes, such as growth, differentiation, nutrition and cleavage of relevant host proteinaceous components.
机译:Fonsecaea pedrosoi是成色菌病的主要病原体,它是一种慢性的,经常使人衰弱的化脓性肉芽肿性真菌病。关于这种人类真菌病原体产生的水解酶知之甚少。在本研究中,我们已经确定了在化学条件下生长时,pedrosoi菌丝体形式的细胞外蛋白水解活性。在体外真菌生长的15天中,使用牛血清白蛋白(BSA)作为可溶性底物和极端酸性pH(2.0),测量了分泌的天冬氨酰肽酶活性。这种活性完全被经典的天冬氨酰肽酶抑制剂pepstatin A抑制。相反,金属(邻菲咯啉),半胱氨酸(E-64)和丝氨酸(PMSF)的蛋白水解抑制剂不能抑制蛋白水解活性。我们还评估了四种不同的HIV天冬氨酰肽酶抑制剂对F. pedrosoi菌丝体分泌蛋白水解活性的影响。茚地那韦,利托那韦和奈非那韦分别有效抑制细胞外天冬氨酰蛋白水解活性,分别约97%,96%和87%,而沙奎那韦并没有明显干扰BSA水解。源自菌丝体的分泌性天冬氨酰肽酶活性可裂解其他蛋白质底物,包括人白蛋白,纤维蛋白原,纤连蛋白,层粘连蛋白和I型胶原。正如我们小组先前报道的那样,分生孢子还可以产生分泌性天冬氨酰肽酶。从这个意义上讲,我们研究了胃酶抑素A对pedrosoi发育的影响。抑肽酶A能够抑制分生孢子的生长及其向菌丝体的转化。两者合计,我们的结果表明天冬氨酰肽酶可能参与基本的真菌过程,例如相关宿主蛋白质成分的生长,分化,营养和裂解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号