Protective responses of wheat treated with fungal pathogens: 2. Activation of anionic peroxidase isoforms in wheat seedlings induced by inoculation with Tilletia caries spores.

摘要

A comparative analysis was conducted of peroxidase isoenzymes from the apical and basal zones of roots and coleoptiles of 4-day-old etiolated seedlings of wheat cv. Zarya resistant to stinking smut (the gene ), and susceptible cultivar Zhnitsa. Theactivity of anionic isoenzymes of peroxidase (AP) including those binding to chitin (chitin-specific) was related to the processes of growth and tissue differentiation. In the basal zone of the root and coleoptile of seedlings, in both resistant and susceptible cultivars, AP was activated following infection by Tilletia caries [T. tritici]. In the resistant cultivar, AP activity was elevated owing to the isoforms AP1 and AP2, which were bound to chitin; in the susceptible cultivar, at the expense of APisoforms, which do not bind chitin, the activity of chitin-specific AP1 decreased.