Structure and Functional Investigation of Ligand Binding by a Family 35 Carbohydrate Binding Module (OCBM35) of β-Mannanase of Family 26 Glycoside Hydrolase from Clostridium thermocellum

摘要

Functional attributes of recombinant QCBM35 (family 35 carbohydrate binding module) of (3-mannanase of family 26 Glycoside Hydrolase from Clostridium thermocellum were deduced by biochemical and in silico approaches. Ligand-binding analysis of expressed CtCBM35 analyzed by affinity-gel electrophoresis and fluorescence spectroscopy exhibited association constants K_a ~ 1.2·10~5 and 3.0·10~5 M~(-1) with locust bean galactomannan and mannotriose, respectively.