Comparison of methods for culturing the reniform nematode, Rotylenchulus reniformis

摘要

The culture of Rotylenchulus reniformis in the liquid Gamborg's B5 medium yielded the highest amount, of 103,389 nematodes per dish, where the solid Gamborg's B5 medium produced 68,371 nematodes per dish. Both methods were more successful than culturing on plants grown in sand. The culture pouches method had the lowest nematode yield. The liquid medium has the advantage to the solid medium in easier handling of egg-mass picking.