Immunoprecipitation mapping of TRX-associated chromosome elements in the fork head gene promoter in Drosophila melanogaster salivary gland cells

摘要

Using the method of immunoprecipitation of the in vivo crosslinked and sheared by sonication chromatin, mapping of potential trithorax-associated regulatory elements within the extended (9 kb) promoter region of the fork head gene (fkh) in the Drosophila melanogaster salivary gland cells was performed. Reaative homogeneity of the salivary gland cells, along with the parallel use of the antibodies to different domains of the same trithorax protein (TRX), and the introduction of cross-hybridization steps for additional specific enrichment of initial DNA libraries, allowed us to improve the method and to identify one major and two less evident potential TRX-binding sites.