It is reviewed opportunities and practical application features of gravimetric biosensors for determination of high-and low-molecular biologically active substances and microorganisms in environments, food stuffs and clinical diagnostics. Characteristics of piezosensors based on different nature receptor molecules (an antibodies, antigens, hapten-protein conjugates etc.) are given. It's reflected the modern view on the wide range of questions, connected with their function in liquids. The special attention is directed towards immunosensors, the most perspective for quantification of trace concentrations in complex matrixes. Possible immunoassay formats in liquid (direct and competitive detection) are given. The questions of formation and regeneration of sensitive layer on the base of biomolecules of different nature are discussed.
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