Dependence of the Formation Function of a Complex with an Oligomer on the Number of Monomers in Its Chain

摘要

Isotherms of ligand adsorption by short oligomers were calcualted in the form of Scatchard dependences by the matrix method and were sued to calcualte ligand-polymer binding constants by the McGhee-Hippel method; the resulting binding parameters are close to theoretical. The complex formation with a polymer can be distinguished from the complex formation with an oligomer statistically, when a precise formation function and physical methods sensitive to the concentrations of monomer forms are available. Parameters of binding 5,10,15,20-tetrakis (1-methyl-4-pyridyl)-21H-porphine with the DNA [d(T_4G_4)]_4 tetraplex at 25 deg C were calcualted on the basis of the matrix and statistical approaches.