Construction of molecular linkage map between Oryza sativa and O. rufipogon, using microsatellite markers

摘要

Several kinds of molecular markers such as RFLP, RAPD, STS, AFLP and microsatellite markers, have been developed and applied in plant genetics and breeding. Of these, microsatellite markers are valuable because they are co-dominant, can detect high levels of allelic diversity and are economically assayed by PCR. In rice, molecular linkage map consisting of more than 500 microsatellite markers are now available to the public (Temnykh et al. 2001). These markers are expected to be efficiently used forQTL analysis and markerassisted selection. In this study, molecular linkage maps between Oryza sativa and O. rufipogon were constructed using microsatellite markers, to confirm the utility of microsatellite markers in studies using wild species of rice.