Reduction of the IgE-binding ability and maintenance of immunogenicity of gamma-irradiated Dermatophagoides farinae

摘要

House dust mites,Dermatophagoides farinae(DF)and Dermatophagoides pteronyssinus,are major allergens in the most common indoor allergen and are important risk factor for asthma.The modified antigen has been studied to treat allergic disorder.This study was carried out to measure possibility of modified allergen using gamma irradiation to treat allergy such as asthma.DF solutions(2mg/ml)as target allergen were irradiated with Co-60 at 50 and 100 kGy.Conformational alternation of irradiated DF was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).Levels of anti-irradiated DF mouse IgGs(sub-isotypes)against intact DF were measured similar to that of anti-intact DF IgGs.The binding abilities of house dust mite-allergic patients' IgE were reduced depending on radiation dose,and irradiation could inhibit the binding ability of patients' IgE more than 40%.This study has shown that the binding ability of IgE was reduced by conformational alteration by irradiation and the irradiated DF had epitopes capable to induce immunogeniciy.