Determination of protein-protein interactions of ICln by the yeast two-hybrid system

摘要

ICIn is a ubiquitously expressed eukaryotic protein. Expression of the protein in Xenopus laevis oocytes, the knocking-down of the protein in fibroblasts, or the reconstitution of the protein in lipid bilayer led to the assumption that this protein is an ionic channel or a significant part thereof. However, other possible roles for ICIn in potential regulatory mechanisms have been postulated, as diverse as regulator of cell morphology by interacting with the Skb1 protein and/or interaction with core spliceosomal proteins. Here we show that ICIn is able to interact with SnRNP core proteins SmD1, SmD2, SmD3, SmX5 and SmB/B'. Copyright (C) 2001 S. Karger AG. Basel. [References: 35]