Activation of NF-kappaB in bone marrow cells of BALB/cJ mice following exposure in vivo to low doses of ~(137)Cs gamma-rays

摘要

We measured levels of NF-kappaB activation in bone marrow(BM)cells collected at 1 and 4 h from male BALB/cJ mice(10-12 weeks old)given a whole body dose of 0,0.05,0.1 and 1 Gy of ~(137)Cs gamma-rays(at the dose rate of 0.75 Gy/min).At each harvest time-point,BM cells were collected from five mice per dose of radiation.We used two methods for detecting NF-kappaB activation(1)the NF-kappaB/p65 transcription factor enzyme-linked immunosorbance assay(ELISA)and(2)immunofluorescence staining with NF-kappaB/p65 antibody.Results from ELISA indicated 2.0 and 2.8-fold increases in NF-kappaB activation in BM cells isolated at 1 h post-exposure of mice to 0.1 or 1.0 Gy.The immunofluorescence staining method showed similar results.In samples isolated 4 h post-irradiation,however,no activated NF-kappaB signal was found,regardless of the method of detection.The data also demonstrated that NF-kappaB was not activated in bone marrow cells collected either at 1 or 4 h from BALB/cJ mice exposed to a single dose of 0.05 Gy ~(137)Cs gamma-rays.Taken together,the results from our in vivo study indicate the involvement of NF-kappaB activation in early response to 0.1 and 1.0 Gy(but not 0.05 Gy)of ~(137)Cs gamma-rays.