Occurrence of tomato spotted wilt virus on three commercial cultivars of Chrysanthemum morifolium : First report in Iran

摘要

Chrysanthemum morifolium (Compositae) is globally the second economically most important floricultural crop following rose, and one of the most important ornamental species. The most affected ornamental crops were surveyed in one main cultivation areain province of Tehran in 2011-12. In all, 116 virus-like symptoms were collected and analyzed by serological DAS-ELISA and TIBA techniques against important diseases of ornamentals, tomato spotted wilt virus (TSWV). The total of 116 ornamental plants were tested, 36 samples were shown to be infected to the virus TSWV. In Tehran province, Bonfire yellow was identified in 28 samples (57.14%), Payton snow in 56 samples (21.42%) and Bella rosa in 32 samples (33.33%). Nucleotide sequences of the N gene of TSWV isolated from infected chrysanthemum were determined. TSWV was identified from chrysanthemum by sequence determination of N nucleocapsid protein, to confirm TSWV identification polymerase chain reaction (PCR) was carried out using samples that previously tested positive by ELISA. All tested commercial cultivars were infected to TSWV. In all, C. morifolium cv. Bonfire yellow was shown to be prevalent TSWV infecting commercial cultivar Chrysanthemum morifolium in these regions. This is the first of natural occurrence of TSWV on three commercial cultivars of C. morifolium in Iran.