Shewanella oshoroensis sp. nov.: A mesophilic eicosapentaenoic acid and hentriacontanonaene-producing bacterium

摘要

A mesophilic marine bacterial strain (strain osh08 T) that produced eicosapentaenoic acid and hentriacontanonaene, was identified by the molecular and ordinary microbiological methods. This strain had the high sequence similarity of its 16S rRNA gene to those of Shewanella basaltis JCM 14937 T (EU143361; 99.8%) and Shewanella hafniensis NBRC 100975 T (AB205566; 98.4%). But, strain osh08 T was phenotypically different from S. basaltis J83 T and S. hafniensis NBRC 100975 T in that it was able to ferment glucose, produce β-glucosidase and β-galactosidase and utilize D-mannose and unable to utilize malate. However, all three strains used iron(III) and manganese(IV) as terminal electron acceptors under anaerobic conditions. The DNA-DNA relatedness between strain osh08 T and S. basaltis J83 T and that between strain osh08 T and S. hafniensis NBRC 100975 T were 4.7% and 11.8%, respectively. The G+C content of strain osh08 T was 43.4 mol%. Strain osh08 T had, in addition to a polar flagellum, many pilus-like structures around the cell that were not observed in the cells of S. basaltis J83 T. Thus strain osh08 T was identified as a new species of Shewanella. The name Shewanella oshoroensis sp. nov. was proposed and strain osh08 T (=NBRC 107685 T) is the type strain.