The effect of manganese-induced toxicity on the cytokine mRNA expression of chicken spleen lymphocytes in vitro

摘要

Manganese (Mn) is essential for life, but excess Mn exposure is harmful. This study investigated the effect of excess Mn on the cytokines of spleen lymphocytes in chicken. Lymphocytes were incubated with or without MnCl2 (2, 4, 6, and 8 x 10(-4) mmol/L) for 12, 24, 36, and 48 h, respectively. The mRNA expression of interleukin (IL) -2, IL-4, IL-6, IL-12 beta, and IL-17 and interferon (INF) -gamma was examined using RT-PCR. Excess Mn inhibited IL-2, IL-4, IL-6, IL-12 beta, and IL-17 mRNA expression in chicken spleen lymphocytes in a dose-dependent manner. IFN-gamma was inhibited by 8 x 10(-4) mmol/L Mn for 48 h. This study demonstrates that excess Mn affects cytokine mRNA expression and causes immunosuppression in chicken spleen lymphocytes. The relationships between IL-6 and IL-17 and between IL-2 and IL-12 beta were strong under immunosuppression caused by excess Mn in lymphocytes. (C) 2015 Elsevier Ltd. All rights reserved.