Relationships between interleukin-12B and interleukin-10 gene polymorphisms and hepatitis C in Chinese Han hemodialysis patients

摘要

Background and Aims: Hepatitis C virus (HCV) commonly causes a chronic infection but portion of hemodialysis patients are able to resistant to HCV infection, even clear the virus naturally. Interleukin-10 (IL-10) and interleukin-12 (IL-12) are crucial regulators of the immune response to T-helper 1 (Th1) or T-helper 2 (Th2) categories and play a role in autoimmune and infectious diseases. This study was undertaken to investigate the possible association of genetic polymorphisms of 3' untranslated region (3'UTR) of the IL-12B (-1188) and IL-10 (-592 and -819) and hepatitis C in Chinese Han hemodialysis (HD) patients. Methods: The genotyping of IL-12B 3'UTR and IL-10 -592 and -819 were performed by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method. Results: Compared with the IL-12B-AA genotype, CC and combined CC/AC genotypes were associated with a significant decreased risk of HCV infection in Chinese hemodialysis patients (p < 0.001). However, the IL-10-592 and -819 genotypes were not found significant difference both between the anti-HCV (+) and anti-HCV (-) patients (p > 0.05) and the persistent infection and viral clearance HD patients (p > 0.05). Conclusions: The present study indicated that the polymorphisms of IL-12B 3'UTR might contribute to the susceptibility of HCV infection in Chinese HD population.