Naydenov et al. found that siRNA-mediated downregulation of aSNAP, a component of the SNARE system, triggers autophagy as measured by the lipidation of LC3 and flux analysis.' The SNARE system participates in vesicular fusion. Its key components include N-ethylmaleimide sensitive factor (NSF) and its adaptor soluble NSF-attachment protein a (aSNAP). Notably, downregulation of NSF had no effects on LC3 lipidation. This suggests that in mammalian cells, the autophagy regulatory function of aSNAP is separated from its other functions that involve its partner protein NSF.
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