DIFFERENTIAL GENE EXPRESSION OF ANGIOTENSIN II RECEPTOR SUBTYPES IN THE EPIDIDYMIDES OF MATURE AND IMMATURE RATS

摘要

Previous studies have suggested that, epididymal and sperm functions are subject to control by a local renin-angiotensin II system (RAS) in the rat epididymis. Type-1 angiotensin II receptor, AT(1) and type-2 receptor, AT(2) were localized in epididymal epithelium, indicating that RAS may act in a paracrine or autocrine fashion to regulate fluid secretion, probably through the basally placed membrane-bound AT(1) protein as revealed by immunocytochemical and electrophysiological studies. In the present work, the expression of the angiotensin II receptor subtypes in the rat epididymis was showed by western blot analysis and reverse-transcription polymerase chain reaction (RT-PCR) using specific primers for the angiotensin II receptor subtypes. Western blot analysis showed the expression of AT(1) receptor in the rat epididymis. Results from RT-PCR, using specific primers based on the corresponding angiotensin II receptor subtype genes for AT(1a) AT(1b) and AT(2), demonstrated the differential expression of mRNAs from these receptor subtypes in the epididymides of mature and immature rats. Both the genes for AT(1a) and AT(1b), but not that for AT(2), are predominantly expressed in the epididymides of mature rat. In contrast, only AT(1a) and AT(2) were highly expressed in the epididymides of immature rat. These results suggest that the expression of type-1 and type-2 angiotensin II receptor subtypes are developmentally regulated. Type-1 subtype may play a role in regulation of electrolyte and fluid transport in mature rat whereas type-2 subtype may be important in growth and development in the immature rat. [References: 24]