Sorption of Gelatin as a Protein Marker on a Porous Substrate of Birch Bark Bast

摘要

The applicability of sorbent made from birch bark bast as a porous substrate to the immobilization of enzymes (catalytic proteins) was studied using the sorption of gelatin as an example. The effects of pH, temperature, and the nature and concentration of supporting electrolytes (NaCl, KCl, CaCl_2, and MgCl_2) on the sorption of gelatin from model solutions were studied. The electrostatic interaction of gelatin with the sorbent surface prevents the adsorption of acidic and alkaline solutions. The sorption capacities of sorbent made from birch bark bast were determined at different gelatin concentrations in a model solution. The maximum sorption capacity for gelatin with the minimum time required to reach equilibrium was observed at pH 4.5.