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Non-contact fluorescent bleaching-independent method for temperature measurement in microfluidic systems based on DNA melting curves

机译:基于DNA熔解曲线的非接触荧光独立于温度的微流体系统温度测量方法

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摘要

This report introduces a bleaching-independent temperature measurement method based on the analysis of the fluorescence emitted during the melting of DNA molecules with the SYBR-Green I intercalator, in a micro volume where the strong non-linearity of the signal is used to eliminate the photobleaching effect as well as to determine the heat transfer rate between a heater and the sample and the temperature non-uniformity within the sample.
机译:本报告介绍了一种独立于漂白的温度测量方法,该方法基于使用SYBR-Green I嵌入剂对DNA分子融化过程中发出的荧光进行的分析,该分析体积很小,信号的强烈非线性可用于消除光漂白作用以及确定加热器和样品之间的传热速率以及样品内的温度不均匀性。

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