Following the Biocatalytic Activities of Glucose Oxidase by Electrochemically Cross-Linked Enzyme-Pt Nanoparticles Composite Electrodes

摘要

An integrated platinum nanoparticles (NPs)/glucose oxidase (GOx) composite film associated with a Au electrode is used to follow the biocatalytic activities of the enzyme. The film is assembled on a Au electrode by the electropolymerization of thioaniline-functionalized Pt NPs and thioaniline-modified GOx. The resulting enzyme/Pt NPs-functionalized electrode stimulates the O_(2) oxidation of glucose to gluconic acid and H_(2)O_(2). The modified electrode is then implemented to follow the activity of the enzyme by the electrochemical monitoring of the generated H_(2)O_(2). The effect of the composition of the Pt NPs/GOx cross-linked nanostructures and the optimal conditions for the preparation of the electrodes are discussed.