A novel strategy employed in identification of 25 important loquat cultivars using RAPD marker

摘要

DNA fingerprinting is both a popular and important technique with great advantages in plant cultivar identification. Despite this fact, this technique has never been used widely and efficiently in practical plant identification since analysis and recording of data generated from fingerprinting and genotyping for future reference is tedious and difficult. We report a novel strategy developed for efficient recording of DNA molecular fingerprints of genotyped plant individuals, which could be readily used as efficient reference information for quick plant identification. 25 orange- or white-fleshed loquat cultivars that are commercially important in China, were identified using this strategy which employed random amplified polymorphic DNA (RAPD) marker. Utilization of the strategy was verified by identification of three randomly chosen groups of loquat culti-vars among the 25. The advantages of this approach include use of fewer primers and separation of all cultivars by the corresponding primers marked on the diagram. Importantly, the diagram could be easily referred to in identification of loquat cultivars in commerial practice.