KINETIC STUDY ON THE FORMATION OF A DE NOVO DESIGNED HETERODIMERIC COILED-COIL - USE OF SURFACE PLASMON RESONANCE TO MONITOR THE ASSOCIATION AND DISSOCIATION OF POLYPEPTIDE CHAINS

摘要

The surface plasmon resonance (SPR) technique was used to study the formation kinetics of a de novo designed coiled-coil (E/K coil), The E/K coil is made up of two distinct peptides (E and K) each with five heptad (g-a-b-c-d-e-f) repeats, The E peptide's heptad sequence is E-V-S-A-L-E-K, and the K peptide's heptad sequence is K-V-S-A-L-K-E, A linker C-nL-G-G-G (nL = norleucine) is present at the C-terminus of the E peptide and at the N-terminus of the K peptide for the SPR studies, Heterodimer formation involves both electrostatic and hydrophobic interactions at the dimer interface, Under conditions that favor the heterodimer formation, the CD signal ([Theta](222)) varied as a function of peptide concentration, The estimated dissociation constant (K-d) was 2.45 +/- 0.71 nM, Denaturation studies with guanidine-HCl (GdnHCl(1/2) = 3.9 M) suggested a value of 3.53 +/- 0.48 nM, For the SPR investigation, the peptides were biotinylated and linked to streptavidin in order to increase their effective molecular weight and consequently enhance the signal intensity, Biotinylation in itself did not impede coiled-coil formation based on CD measurements, The biosensor study revealed a slow dissociation rate constant for the heterodimer (k(d) approximate to 2 x 10(-4) s(-1)) and a moderately fast association rate constant [k(d) approximate to (4.27-4.53) x 10(5) M(-1) s(-1)), This gives a calculated K-d of 0.47-0.50 nM, which agrees reasonably well with the equilibrium CD studies, Therefore, based on the SPR data, the preference for heterodimer formation is due to a combination of moderately fast association and slow dissociation rates.