In vitro Shoot and Callus Induction in Brahmi

摘要

Plants of Brahmi Bacopa monnieri (L.) Pennell were regenerated by taking explants isolated from field-grown mature plants. This was achieved by culturing on MS solid medium with and without growth hormones. The best medium for initiation and development was MS+4 mg/l BAP +75 mg/l Adenine sulphate where the percentage of establishment was found to be very high (100%). In the multiplication stage MS +4mg/l BAP medium was found to be best in terms of average number of shoots (5.25). In the rooting stageMS+1.5mg/l NAA was found to bethe best medum in terms of average number of roots (7.5). The medium MS + 1 mg/2, 4-0 was the best medium for callus induction. This protocol can be used to generate cost-effective protocol for large-scale in vitro multiplication of brahmi.