Effects of deoxynivalenol (DON), zearalenone (ZEN), and related metabolites on equine peripheral blood mononuclear cells (PBMC) in vitro and background occurrence of these toxins in horses

摘要

Both deoxynivalenol (DON), zearalenone (ZEN), and their metabolites are known to modulate immune cells in various species whereby viability and proliferation are influenced. Such effects were rarely examined in horses. Therefore, one aim of the present study was to titrate the inhibitory concentrations of DON, 3-acetyl-DON (3AcDON), de-epoxy-DON (DOM-1), ZEN, and a- and |3-zearalenol (ZEL) at which viability and proliferation of equine PBMC were reduced by 50 % (IC5o) and 10 % (IC_0) in vitro. For evaluation of practical relevance of the in vitro findings, a further aim was to screen horses for the background occurrence of DON, ZEN, and their metabolites in systemic circulation and to relate toxin residues both to the inhibitory toxin concentrationsand to hematological and clinical-chemical characteristics.The IC_(50) (uM) for DON, 3AcDON, 3-ZEL, cc-ZEL, and ZEN were determined at 3.09, 25.90,75.44, 97.44, and 98.15 in unstimulated cells, respectively, while in proliferating cells, the corresponding IC_(50) values were 0.73, 6.89, 45.16, 75.96, and 82.51.Neither viability nor proliferation was influenced by DOM-1 up to a concentration of 100 uM.The in vivo screening (N= 49) revealed the occurrence of ZEN (N=2A), a-ZEL (N=3), (3-ZEL (N=37), DON, and DOM-1 (N=2). The detected concentrations were much lower than the corresponding IC50 while the ICi0 of DON and (3-ZEL for proliferating PBMC corresponded to approximately 26 and 35 ng/mL which might be relevant when contaminated diets are fed.Clinical-chemical and hematological traits were not related to mycotoxin residue levels excepting blood urea nitrogen which was positively correlated to the sum of (3-ZEL, a-ZEL, and ZEN concentration. Whether this reflects simply the feeding history ofthe horses or renal failures giving rise to a prolonged half-life of the toxins needs to be clarified further.