spatial coordination between cell and nuclearshape within micropatterned endothelial cells

摘要

Growing evidence suggests that cytoplasmic actin filaments are essential factors in themodulation of nuclear shape and function. However, the mechanistic understanding of theinternal orchestration between cell and nuclear shape is still lacking. Here we show thatorientation and deformation of the nucleus are regulated by lateral compressive forces drivenby tension in central actomyosin fibres. By using a combination of micro-manipulation tools,our study reveals that tension in central stress fibres is gradually generated by anisotropic forcecontraction dipoles, which expand as the cell elongates and spreads. our findings indicate thatlarge-scale cell shape changes induce a drastic condensation of chromatin and dramaticallyaffect cell proliferation. on the basis of these findings, we propose a simple mechanical modelthat quantitatively accounts for our experimental data and provides a conceptual framework forthe mechanistic coordination between cell and nuclear shape.