OM-X (R), Fermented Vegetables Extract Suppresses Antigen-Stimulated Degranulation in Rat Basophilic Leukemia RBL-2H3 Cells and Passive Cutaneous Anaphylaxis Reaction in Mice

摘要

OM-X (R) is a hand-made and naturally manufactured probiotic supplement. This fermented food product is made from vegetables, fruits, seaweeds and mushrooms, using 12 strains of lactic acid bacteria and bifidobacteria. OM-X (R) is also known to have beneficial health properties, and some of its components show effects on antigen (Ag)-stimulated degranulation activity, indicating that OM-X (R) may be useful in the treatment of allergy responses and symptoms. In this study, we evaluated the inhibitory effects of OM-X (R) on Ag-stimulated degranulation in rat basophilic leukemia RBL-2H3 cells, clarified the underlying mechanisms, and determined the active compounds in OM-X (R) for suppression of degranulation. Treatment with OM-X (R) gradually suppressed Ag-stimulated degranulation throughout the maturation period. OM-X (R) also gradually produced melanoidins by lactic acid bacterial fermentation during the maturation process. There was a high correlation between the suppression levels of Ag-stimulated degranulation and the browning of OM-X (R). Furthermore, the inhibition of Ag-stimulated degranulation by OM-X (R) was found to be partially due to the direct inactivation of NADPH oxidase. To elucidate the in vivo effects of OM-X (R), type I allergy model mice were orally administered with OM-X (R) and the passive cutaneous anaphylaxis (PCA) reaction was measured. OM-X (R) intake remarkably suppressed the PCA reaction. Taken together, our findings suggest that OM-X (R) could be a beneficial food to ameliorate allergic reactions.