Natural Bioenergetics Systems of Halobacteria Halobacterium salinarum for Nanobiophotonics

摘要

new method for preparing the naturally orientated cellular energytransducing membranes of halobacteria Halobacterium salinarum (NOPM) is offered. This method makes it possible to simplify the technique of extracting the immobilized purple membranes of halobacteria and to obtain photosensitive membrane preparations of a vast area. If the tubular substrates were used, closed photosensitive NOPM struc tures similar to the natural ones were created. Methods for atomic force microscopy were first revealed on an internal surface of membranes of halobacterial cells raised in optimal conditions in a reactor; the purple membranes were surrounded by the sites enriched with the molecules of ATPsynthase. Thus, in their natural complex, this method makes it possible to obtain preparations of these immobilized membranes with key functional transmembrane proteins of two bioenergetics systems of halobacteria containing (i) purple mem branes as the acceptor part and the lightdriven proton pump for proton gradient generation and (ii) a storing part as transmembrane complexes for the ATP synthesis. NOPM preparations can be used in nanobiophoto nics for the creation of artificial photoelectric devices for multipurpose applicat