New Polymeric Nanosorbents for Selective Binding of Biological Macromolecules

摘要

Acrylamide-based hydrogel nanoparticles, which had hydrodynamic radii in aqueous dispersions of about 100–180 nm, were prepared by miniemulsion polymerization. The redox initiation at the interfacial boundary made it possible to conduct synthesis at an ambient temperature with a high rate and resulted in latexes of enhanced aggregative stability. The original method for nanoparticle functionalization by aminophenylboronic acid was elaborated for their application as affinity sorbents for 1,2-diols. The ability to reversibly bind ribonucleoside (inosine) and glycated protein (hemoglobin) has been demonstrated. The binding capacity relative to inosine and glycated hemoglobin was determined as a function of the phenylbo-ronate group content.