Identification and Cliaracterization of Alginate Lyase Producing Pseudomonas sp. N7151-6

摘要

A Gram-negative, alginate lyase-producing bacterium was isolated from the Haeundae Coast, Korea. The isolated strain N7151-6 produced alginate lyase. The optimal temperature and pH for growth were found to be 30 de C and pH 8.0, respectively. This strain can be grown at the NaCl concentration of 0-7% (w/v). Analysis of 16S rDNA sequence and physiological profiling indicated that the strain N7151-6 belonged to Pseudomonas sp. The enzyme alginate lyase produced by Pseudomonas sp. N7151 -6 was partiallypurified by ultrafiltration (MWCO= 30 kDa). The optimum pH and temperature for the activity of the purified enzyme were found to be 7.0 and 30 deg C, respectively. The enzyme was stable at the pH range of 5.0-9.0 and temperature range of 23-37 deg C. The total activity of alginate lyase produced was reached about 110 unit/L.