Evaluation of Genetic Relationships among Persimmon Cultivars Introduced and Indigenous in Korea Using RAPD

摘要

The genetic relationships among 60 oriental persimmon (Diospyros kaki Thunb.) accessions including 30 pollination-constant non-astringent (PCNA), 16 pollination-variant non-astringent (PVNA), 6 pollination-variant astringent (PVA), and 8 pollination-constant astringent (PCA) cultivars were evaluated using 39 RAPD (randomly amplified polymorphic DNA)-PCR primers. A total of 185 polymorphic bands out of 250 RAPD bands scored were obtained and unique fingerprints for all 60 cultivars were produced, despite inclusion of closely related bud-sport cultivars. Pair-wise genetic similarity coefficient (Nei-Li) among all pairs of 60 cultivars varied from 0.62 (between Taishu' and 'Saijo') to 0.99 (between 'Superhiratanenashi' and 'O-tanenashi'). Unweighted pair-group method with arithmetic averaging (UPGMA) clustering analysis revealed two main clusters, I and II; all 30 PCNA cultivars formed cluster I and showed a narrow genetic diversity among themselves (0.85-0.99). Cluster II contained PVNA cultivars andother astringent type cultivars. Principle component analysis (PCA) showed a third group consisting of seven PVA cultivars, in addition to cluster I and II that were revealed by UPGMA clustering. RAPD-based phenetic relationships among the persimmon cultivar were comparable to known pedigree records, morphological observations, and reports from previous DNA fmgerprinting studies that used different molecular marker types. Our study demonstrated that RAPD markers can be efficiently used for genetic diversity assessment of closely related persimmon varieties and cultivar identification, which are essential for modern breeding program.