Isolation and identification of cellulose-producing bacteria

摘要

Extensive screening for cellulose-producing bacteria was done using differential media. Fifty seven strains were isolated totally from the fruits and the vinegar, respectively; the isolate A9 strain from apples was selected and examined to determine its taxonomical characteristics. The bacterium was identified as the genus Acetobacter sp. based on morphological, cultural and biochemical properties. A9 strain produced acetic acid from ethanol and decomposed acetic acid to CO_2 and H_2O. They produced dihydroxyacetone from glycerol but did not produce #gamma#-pyrone from glucose and fructose. When A9 strain was cultivated statically in Hestrin and Schramm liquid medium(HS medium), thick cellulose pellicle was formed. Higher cellulose production was obtained in the shaken culture using HS medium at 100 rpm.