TLR9 agonist enhances lung cancer invasiveness by alternating miRNA expression profile

摘要

o investigate the role of miRNA expression profile in the increased invasiveness of non-small cell lung cancer (NSCLC) 95D cells upon TLR9 agonist stimulation, miRNA microarray assay was performed to detect the expression profile of miRNA in NSCLC 95D cells with or without treatment of CpG oligodeoxynucleotide (ODN). Real time PCR was performed in twenty NSCLC samples and corresponding normal tissues to verify the expressions of target miRNA. The miRNA microarray assay showed that CpG ODN stimulation alternated the miRNA expression profile in NSCLC 95D cells and the difference in the expressions of 23 miRNAs between the CpG ODN treated group and untreated group was at least two-fold, among which 20 miRNAs were down-regulated. The down-regulation of let-7a was the most significant, which was also confirmed by Real time PCR. We concluded that TLR9 agonist might raise the invasiveness of NSCLC 95D cells by alternating the expression profile of miRNA, especially the down-regulation of let-7a.