首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Chromosomal aberrations, sister chromatid exchanges and micronuclei induced by pentoxifylline in in vitro cultivated Chinese hamster cells (V79) and human blood lymphocytes.
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Chromosomal aberrations, sister chromatid exchanges and micronuclei induced by pentoxifylline in in vitro cultivated Chinese hamster cells (V79) and human blood lymphocytes.

机译:己酮可可碱在体外培养的中国仓鼠细胞(V79)和人血淋巴细胞中引起的染色体畸变,姐妹染色单体交换和微核。

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摘要

Pentoxifylline (PTX) is a methylxanthine widely used in clinical practice. The mechanism of PTX effects on cellular and molecular level have not been fully explained yet. The present study was carried out to investigate the cytogenetic effect of this drug using cultured Chinese hamster V79 cells and human blood lymphocytes in vitro. The occurrence of chromosomal aberrations (CA), sister chromatid exchanges (SCE) and micronuclei (MN) was observed after the treatment of cells by different concentrations (0.002-2.0mg/ml) of PTX. In exposed V79 cells and lymphocytes as well, the dose-dependent increases of the above mentioned cytogenetic endpoints were found. The statistically significant increase has appeared at lower PTX concentrations in human lymphocytes than in V79 cells in all the investigated parameters.Our results show that, the applied concentrations of PTX has the clastogenic effect on in vitro cultured V79 cells and human lymphocytes. These findings are notable because of the frequent use of this drug and may serve as preliminary data to the further detailed examination of PTX action on molecular level.
机译:己酮可可碱(PTX)是一种广泛用于临床实践的甲基黄嘌呤。 PTX影响细胞和分子水平的机制尚未完全阐明。本研究旨在利用体外培养的中国仓鼠V79细胞和人血淋巴细胞研究该药物的细胞遗传学作用。用不同浓度(0.002-2.0mg / ml)的PTX处理细胞后,观察到染色体畸变(CA),姐妹染色单体交换(SCE)和微核(MN)的发生。同样在暴露的V79细胞和淋巴细胞中,发现了上述细胞遗传学终点的剂量依赖性增加。在所有研究参数中,人淋巴细胞中的PTX浓度均比V79细胞中的PTX浓度低时,具有统计学上的显着增加。我们的结果表明,施用的PTX浓度对离体培养的V79细胞和人淋巴细胞具有致死作用。这些发现是值得注意的,因为该药物的频繁使用,可以作为进一步详细研究PTX分子水平作用的初步数据。

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