Preparation and identification of a novel antibody against human CDC7 kinase

摘要

Cell division cycle 7-related protein kinase (CDC7), which is conservatively expressed in the eukaryotic cells, is being intensely studied because of its significant function in DNA replication. In order to get further information on human CDC7, we generated a novel antibody against human CDC7. The steady strain of hybridoma (2G12) that can secrete specific monoclonal antibodies against human CDC7 was obtained by hybridoma technique. It is poised to contribute novel ways to study the cell cycle. The isotope of the monoclonal antibody was tested to be IgG2a/κ, and its characterizations were shown by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. The affinity constant (Kaff ) of the monoclonal antibody was measured by non-competitive ELISA. By Western blot analysis, we found that CDC7 was largely expressed on the HCCLM3 cell line. Further identifications were adopted by the HRP-labeled MAbs. Thus, the antibody might boost studies on tumor cell lines.