Isolation of highly polymorphic autosomal microsatellite loci and a sex-linked locus from sugarbirds

摘要

We describe the isolation of six tetranucleotide microsatellites from the Cape sugarbird (Promerops cafer) using an enrichment protocol. All loci were highly variable with number of alleles ranging from nine to 26 and values of observed heterozygosity ranging from 0.534 to 0.931. All loci were in Hardy-Weinberg equilibrium with the exception of Pro66 and Pro86. Further analysis of Pro86 indicated it was Z-linked. All loci amplified and were variable in the congeneric Gurneys sugarbird (Promerops gurneyi). These loci will be used in mating system and phylogeographic studies of Cape sugarbirds in South Africa.