SiO2@antisense molecules covered by nepetalactone, extracted from Nepeta gloeocephala, inhibits ILK phosphorylation and downstream PKB/AKT signaling in HeLa cells

摘要

In this study, the anticancer property of SiO2@antisense molecules (SiO2@AMs) and SiO2@AM covered by nepetalactone (SiO2@AM/CN), extracted from Nepeta gloeocephala, was investigated. Here integrin-linked kinase (ILK) phosphorylation and protein kinase B/AKT (PKB/AKT) signaling was Studied when HeLa cells were exposed to SiO2@AM and SiO2@AM/CN. First; N. gloeocephala was identified at the Iranian National Herbarium. Then, its essential oil (EO) was obtained by the hydrodistillation method. In the next, step, 4a alpha,7 alpha,7a alpha-nepetalactone Was extracted from the EO, based on the spectroscopic data. To obtain SiO2@AM/CN, 1 ml of SiO2@AM was mixed with extracted nepetalactone and then strongly shaken for 30 min. Finally, serial concentrations (100, 50, 25 and 12.5 mu g ml(-1)) of SiO2@AM and SiO2@AM/CN; were prepared and then exposed to HeLa cells (2x10(5) cells per ml), for 24 h at 37 degrees C. After incubation; 3-(4,5-dimethylthiazol-2-yl),-2,5-diphenyltetrazoliurn bromide (MTT) assay, cell-cycle analysis, terminal deoxynucleotidyl trangerate dUTP nick-end labeling (TUNEL) assay and western blots were carried out. To find ILK phosphorylation PKB/AKT signaling, the expression of threonine-173 (Thr-173), serine-246 (Ser-246), total ILK, AKT-Ser473, AKT-Thr308 and total AKT was investigated. HeLa cells that were treated with SiO2@AM/CN had G2/M arrest. Based on the TUNEL assay, many apoptotic cells have been shown when they were exposed to SiO2@AM/CN. Importantly, SiO2@AM/CN decreased ILK phosphorylation at Thr-173 and Ser-246 without affecting total ILK levels. Moreover, SiO2@AM/CN decreased AKT-Ser473: and AKT-Thr308 phosphorylation without affecting total PKB/AKT protein.