首页> 外文期刊>Metallomics. integrated biometal science >Distribution of trace elements in the mammalian retina and cornea by use of particle-induced X-ray emission (PIXE): localisation of zinc does not correlate with that of metallothioneins
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Distribution of trace elements in the mammalian retina and cornea by use of particle-induced X-ray emission (PIXE): localisation of zinc does not correlate with that of metallothioneins

机译:通过使用粒子诱导的X射线发射(PIXE),在哺乳动物的视网膜和角膜中微量元素的分布:锌的定位与金属硫蛋白的定位不相关

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Proton-induced X-ray emission (PIXE) in combination with 3D depth profiling with Rutherford backscattering spectrometry (RBS) was used to establish the distribution and concentration of trace elements within individual corneal and retinal areas in frozen sections from adult male Wistar rats (n = 6). The distribution of endogenous trace elements in the cornea and retina is non-homogenous. The most abundant metal in the cornea is calcium followed by zinc. Iron and copper are present in small amounts localised particularly to the epithelium. Iron is also identified in keratocytes. Relatively high levels of calcium occur in the corneal epithelial cell bodies. Zinc has a wide intense distribution across the corneal epithelium (with greater levels in the basal part) and posterior stroma. In the retina, zinc is the most common metal followed by iron and copper. Relatively high levels of zinc exist in the retinal pigment epithelium (RPE), photoreceptor inner segments (RIS) and inner nuclear layer (INL). Chelatable zinc was localised with fluorescent TSQ in the RPE, RIS and plexiform layers. It is interesting to note that the highest levels of total zinc and the greatest intensity of chelatable zinc staining do not coincide. In the RPE and corneal epithelium, zinc co-localised with the zinc-containing metallothioneins (MT). However, there was a clear mismatch between the localisation of the most intense levels of zinc in the neuroretina (i.e. INL) and corneal posterior stroma with that reported for MT. For example, the presence of zinc is not particularly associated with the retinal ganglion cells, retinal area that contains MTs in significant amounts. While high amounts of zinc are present in the INL and corneal posterior stroma, which are largely devoid of MTs. This probably represents pools of static, catalytic and structural zinc associated with substances other than the MTs.
机译:质子诱导的X射线发射(PIXE)结合卢瑟福反向散射光谱(RBS)的3D深度剖析用于确定成年雄性Wistar大鼠冰冻切片中单个角膜和视网膜区域内微量元素的分布和浓度(n = 6)。内源微量元素在角膜和视网膜中的分布是不均匀的。角膜中最丰富的金属是钙,其次是锌。铁和铜以少量存在,特别是位于上皮细胞中。铁还存在于角膜细胞中。角膜上皮细胞体中钙的含量相对较高。锌在角膜上皮(基底部分的含量较高)和后基质中分布广泛。在视网膜中,锌是最常见的金属,其次是铁和铜。视网膜色素上皮(RPE),感光细胞内部片段(RIS)和内核层(INL)中存在相对较高的锌含量。可螯合的锌通过荧光TSQ定位在RPE,RIS和丛状层中。有趣的是,最高的总锌含量和最大的可螯合锌染色强度并不一致。在RPE和角膜上皮中,锌与含锌金属硫蛋白(MT)共定位。但是,神经视网膜(即INL)和角膜后基质中最强水平的锌的定位与MT报道的锌之间明显不匹配。例如,锌的存在与视网膜神经节细胞并不特别相关,视网膜神经节细胞含有大量的MT。虽然INL和角膜后基质中存在大量的锌,但大部分没有MT。这可能代表与MT以外的物质相关的静态,催化和结构性锌的集合。

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