Interaction Studies of Anticancer Drug Lomustine with Calf Thymus DNA using Surface Enhanced Raman Spectroscopy

摘要

Drug-DNA interaction study plays a significant role in understanding the drug action at molecular level and hence rationale drug designing. Lomustine (1-[2-chloroethyl]-3-cyclohexyl-1-nitroso-urea) is an alkylating antineoplastic nitrosourea derivative, used to treat various types of cancer. In the present investigation, conformational and structural effects of lomustine on DNA structure are analyzed using surface enhanced Raman spectroscopy. Different drug/DNA molar ratios are studied to determine the binding sites and binding mode of lomustine with DNA. Results suggest binding of lomustine with nitrogenous bases guanine and cytosine alongwith weak interaction to the sugar-phosphate backbone of DNA. The present study may support rational drug designing efforts to develop nitrosourea based novel therapeutics.