Use of 16S-rRNA based techniques to investigate the ecological succession of microbial populations in the immature lamb rumen: tracking of a specific strain of inoculated Ruminococcus and interactions with other microbial populations in vivo.

摘要

The establishment of microorganisms in the rumen is a critical step if rumen manipulation is to be accomplished by use of microbial inoculants. Microbial populations in the maturing rumen undergo successional changes and, while in a state of flux, provide a possible opportunity for the introduction of specific strains of bacteria. While the rumen of the young lamb was maturing, we measured changes in several microbial populations with 16S-rRNA specific oligonucleotides: Ruminococcus, Fibrobacter, eukaryotes, Gram-positive bacteria, the Bacteroides-Porphyromonas-Prevotella group, and anaerobic rumen fungi. In this study we repeatedly dosed 15 lambs with approximately 3.4X108 to 0.8X109 Ruminococcus cells dose-1, twice a week, for 7 weeks from 23 to63 days of age. Of the five Ruminococcus strains dosed (R. albus SY3 and AR67, and R. flavefaciens Y1, LP9155, and AR72) the most specific primers (based on 16S rDNA) were obtained for strain SY3. There was an increase in the eukaryotic population during dosing, and it was hypothesized that protozoal predation contributed to the disappearance of strain SY3. At the end of dosing PCR amplification showed that SY3 were approximately 109 cells ml-1, but decreased to below the detection limit of the PCR system (8.6X104 ml-1) within 28 days postdosing. These experiments showed that fibrolytic populations increased (P<0.1) above the controls during the dosing period and were elevated for several days postdosing. This suggests that dosing of highly fibrolyticbacteria makes more of the fibre available to other organisms able to degrade fibre, and in so doing increases the overall fibrolytic activity of the rumen. Examination of the succession of gram-positive bacteria and the Bacteroides-Porphyromonas-Prevotella group showed a decline in relative abundance as the lambs matured.