A quantitative approach to cytoarchitectonics

摘要

The cerebral cortex of the guinea pig has been examined by means of a quantitative cytoarchitectonic method (Schleicher et al. 1978; Zilles et al. 1978a). In this method, a computer-controlled automatic image analyzer determines the grey level index of microscopic fields measuring 50x50 μm in Nissl-stained sections by a systematic scanning procedure. Computer plots of serially sectioned histological slides from three hemispheres were produced by printing selected ranges of grey level indices. The delineation of cortical areas was worked out in these plots based on quantitative criteria. Cortical maps of the areal pattern were reconstructed graphically