Breakdown of phosphatidylinositol in soybean callus

摘要

We have investigated the breakdown of membrane-bound phosphatidylinositol (PI) in homogenates of soybean (Glycine max) callus. The breakdown of PI was stimulated by the detergent deoxycholate. At pH 7.0 and 1·gl-1of deoxycholate the loss of PI was rapid and extensive: more than 80 was broken down within 10 min. The breakdown of PI was also stimulated by millimolar concentrations of Ca2+. The products of breakdown of added PI (purified from soybean callus) in this system were identified from their chromatographic mobilities as 1,2-diacylglycerol,myo-inositol 1-phosphate andmyo-inositol 1:2-cyclic monophosphate