Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization

摘要

Nonradioactive in situ hybridization with an α-satellite DNA probe specific for chromosome 18 was performed on human interphase sperm nuclei to detect the frequency of sperm cells disomic for chromosome 18. A total of 16127 sperm heads from eight healthy donors, aged 23–57 years, was investigated, and a minimum of 2000 sperm nuclei per proband was analyzed. The disomy rate ranged from 0.25 to 0.5, with an average of 0.36. This frequency does not differ significantly from that determined for other chromosom