Changes in Soluble and Bound Peroxidase—IAA Oxidase During Tomato Fruit Development

摘要

ABSTRACTSoluble peroxidase activity increased dramatically during the early stages of tomato fruit development, reaching a maximum at the mature‐green stage. Ionically and covalently bound peroxidases were also observed, and the activities of these fractions increased steadily throughout fruit development. IAA (indoleacetic acid) oxidase activity was observed in both soluble and bound fractions and paralleled the peroxidase activity. Tissue homogenates from juvenile fruit caused an extended induction period in IAA oxidation reactions catalyzed by the purified tomato fruit peroxidase, suggesting the occurence of a high concentration of phenolic‐type auxin protectors in this tissue. As the fruit developed, tissue homogenates showed a reduction in the amount of auxin protectors as the IAA oxidizing capacity of the fruit increa